Biophotonics in integrated optofluidic devices

Optofluidics defines a wide research field that is characterized by the synergic combination of optics and microfluidics. In biophotonic applications, optofluidic is generally exploited to increase the miniaturization of the devices towards integrated platforms called Lab-on-chips. These are microsystems aiming at the miniaturization onto a single substrate of several functionalities that would usually require an entire biological laboratory. The technique of femtosecond laser irradiation followed by chemical etching (FLICE) proved to be ideally suited for lab-on-chip fabrication as it provides the integration of both microfluidic and optical functions on the same glass chip leading to monolithic, perfectly aligned, robust and portable optofluidic devices. 


This research line is dedicated to the development and implementation of integrated optofluidic devices for different biophotonic applications. 

People involved

Roberto Osellame
Roberta Ramponi
Petra Paiè
Tommaso Zandrini

In particular, integrated fluorescence and label-free sensing of biomolecules in a lab-on-a-chip has been demonstrated.  The combination of microfluidics and optical tweezers technologies allowed the fabrication of chips for single cell manipulation, trapping and stretching. These functionalities have been succesfully demonstrated both by the inscription of optical waveguides in commercial microfluidic chips and by the fabrication of both optical and fluidic components through FLICE technique. Recently, the activity has been focused on the demonstration of a micro fluorescence activated cell sorter for single cell sorting and collection.


Collabor
ations: M. Pollnau (University of Twente), H. van den Vlekkert (LioniX bv), R. van Weeghel (Zebra Bioscience bv), P. Watts (Hull University), I. Cristiani (Università di Pavia), J. Guck (BIOTEC - Technische Universität Dresden), B. Hecht (Wuerzburg University).